Biotin-Control Oligo

Biotinylated Positive Control Oligo Probe (ALU)

patents pending

Technical specifications

: 0.8 ml probe solution for 25 assays.

: the probe is supplied in a ready-to-use form optimally diluted in hybridization mixture.

Application : as a positive control probe in DNA in situ hybridization techniques on

paraffin sections, frozen sections and cytological specimen.

Recommended use : allow reagent to warm up to room temperature (20-30°C) before starting.

Dehydrate digested specimens in 100% ethanol for 10 sec. and airdry. Apply one drop of probe solution per specimen and cover with a coverslip. Place the slides on a 95°C hotplate and incubate for 5 min.

Incubate the slides for 2 hrs at 37°C. Gently remove coverslips by soaking slides in TBS buffer for 10 min. Wash the slides for 3 x 1 min. in TBS buffer and start detection procedure.

Size of probe : mixture of six 30-meric oligonucleotides complementary to ALU repeats. Purification : performed by size exclusion chromatography. Detection limit : at least 10-30 pg by filter hybridization using PanPath's biotin detection

system (product code: DSB18).

Storage : store refrigerated at 2-6°C. Stability : at least stable until expiry date printed on label. Precautions : - homogenize probe solution before usage. - avoid contact with eyes and skin. Quantity Format

References

1. Autillo-Touati A, Acta Cytologica, Vol. 5, No. 3, p. 631-638 (1998). 2. Jelsma T, Journal of NIH Research Vol. 5, p. 82 (1994).

Related products

TBS buffer product code: BC0017

Please contact your local supplier for further information.

1.

The probes in this product are labeled with the Universal Linkage System (ULS). This product or the use of this product may be covered by one or more patents of

KREATECH Biotechnology BV, including, but not restricted to, the following: EP 0539466; US 5,580,990; US 5,714,327; WO 92/01699; WO 96/35696; WO 98/155.

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