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Methods and apparatus for amplifying nucleic acids

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专利名称:Methods and apparatus for amplifying

nucleic acids

发明人:Haswell, Stephen John,Shaw, Kirsty

Jane,Docker, Peter

申请号:AU2011306965申请日:20110921公开号:AU2011306965B2公开日:20150618

摘要:A method of amplifying nucleic acid comprises providing a microfluidic device(10) having a space therein (32). The space (32) is filled with a gel medium (). Reagentsfor carrying out a PCR reaction are supported within the matrix of the gel medium ()within the space (32). A nucleic acid containing sample is brought into contact with the gelmedium (). The sample comprises whole cells or cell lysate without any priorseparation of the nucleic acid from other components of the cells. PCR amplification ofnucleic acid from the sample is performed in the space using the PCR reagents.

申请人:The University of Hull

代理人:Shelston IP

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