专利名称：PROMOTER-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE
发明人：David Frendewey,Guochun Gong,Ka-Man
Venus Lai,David M. Valenzuela
申请号：US14571515申请日：20141216
公开号：US20150099296A1公开日：20150409
摘要：Targeting constructs and methods of using them are provided for
differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to arecombinase are provided, wherein the promoter drives transcription of the recombinasein an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1,Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked onboth sides by recombinase sites can be removed using a recombinase gene operablylinked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed inundifferentiated cells but not in differentiated cells. The constructs may be included intargeting vectors, and can be used to automatically modify or excise a selection cassettefrom an ES cell, a non-human embryo, or a non-human animal.
申请人：REGENERON PHARMACEUTICALS, INC.
地址：TARRYTOWN NY US
国籍：US
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