Method and apparatus for making porous agarose bea

专利名称：Method and apparatus for making porous

agarose beads

发明人：Kwok-Shun Cheng,Senthilkumar

Ramaswamy,Nanying Bian,BrianGagnon,Joaquin Umana,Neil P. Soice

申请号：US11520775申请日：20060913公开号：US07678302B2公开日：20100316

专利附图：

摘要：The present invention relates to a method and apparatus for forming agarose

or cored agarose beads. The process involves dissolving/gelation the agarose in asuitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintainingthat emulsion at a temperature equal to or greater than the gelation point of theagarose, passing it through a static mixer to create agarose droplets and solidifying theagarose droplets in a second bath of hydrophobic liquid. The beads can then be washedand used or further processed to crosslink the agarose and/or add various functionalitieson to the agarose. Another method for solidifying the agarose droplets is by using a heatexchanger to cool the stream continuously after it exits the static mixer. A similar processis used for the “cored” beads except cores, preferably in bead form, are introduced tothe agarose before it enters the first hydrophobic liquid so that the agarose forms acoating on the cores. A similar process with either agarose beads (made by this oranother process) or cored agarose (made by this or another process) can be used to addmultiple layers of agarose on to the existing beads. An apparatus for running the processis also disclosed.

申请人：Kwok-Shun Cheng,Senthilkumar Ramaswamy,Nanying Bian,BrianGagnon,Joaquin Umana,Neil P. Soice

地址：Nashua NH US,Nashua NH US,Nashua NH US,Billerica MA US,StoneHame MAUS,Amherst NH US

国籍：US,US,US,US,US,US

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